| 教員紹介トップページへ | 三重大学トップページへ |
| 職名 | 教授 | |||||||||
| 氏名 | たけばやし しんいちろう 竹林 慎一郎 |
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| 生年月 | 1974.09 | |||||||||
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| TEL | 059-231-9617 | |||||||||
| FAX | 059-231-9617 | |||||||||
| stake@bio.mie-u.ac.jp | ||||||||||
| 個人のホームページ | https://katsu2634molcell.wixsite.com/molcellbio | |||||||||
| 学歴 | 1997年３月 三重大学生物資源学部 卒業
1999年３月 三重大学大学院生物資源学研究科 修士課程 修了 2002年３月 三重大学大学院生物資源学研究科 博士課程 修了 |
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| 学位 | 博士（学術） | |||||||||
| 所属学会 | 日本分子生物学会
日本エピジェネティクス研究会 日本農芸化学会 |
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| 社会活動 | ||||||||||
| 職歴 | 1999年４月- 2002年３月 独立行政法人・日本学術振興会・特別研究員 (DC1)
2002年４月- 2007年9月 独立行政法人・神戸理化学研究所・発生再生科学総合研究センター・研究員 2007年10月 – 2012年6月 米国フロリダ州立大学・生物科学部門・ポストドクトラルフェロー 2012年7月 – 2013年4月 熊本大学発生医学研究所・研究員 2013年5月 – 2014年3月 熊本大学大学院先導機構・特任助教 2014年4月 - 2019年2月 三重大学大学院医学系研究科・講師 2019年3月 - 2023年7月 三重大学大学院生物資源学研究科・准教授 2017年5月 - 現在 国立研究開発法人・理化学研究所・客員研究員（兼任） 2023年8月 - 現在 三重大学大学院生物資源学研究科・教授 |
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| 学術（芸術）賞 | 三重大学優秀論文・著書・作品賞論文賞,2022.03,国立大学法人三重大学
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| 専門分野 | エピジェネティクス | |||||||||
| 現在の研究課題 | 遺伝子の機能的発現を可能にする高次染色体構造制御
１細胞ゲノム網羅的解析技術の開発 老化におけるエネルギー代謝変化とエピジェネティクス |
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| 担当科目 | 卒業研究，生化学Ⅱ，生命機能化学実験実習5/6，化学基礎II， 化学基礎II（再履修クラス），生命機能化学演習 I/II，生物圏生命化学概論，生物資源学総論，分子細胞生物学特論，分子細胞生物学演習，生命機能化学特別研究 I/II，生命機能化学特論Ⅰ | |||||||||
| 主な業績等 | （学術論文，* Corresponding author）
Hayakawa, T., Yamamoto, A., Yoneda, T., Hori, S., Okochi, N., Kagotani, K., Okumura, K., and *Takebayashi, S. (2023) Reorganization of the DNA replication landscape during adipogenesis is closely linked with adipogenic gene expression. J. Cell Sci.136: jcs260778 Tsuji, T., Ikuno, A., Hayakawa, T., Katsuzaki, H., Takebayashi, S., Kagotani, K., and Okumura, K. (2022) Hexane Extract of Citrus sphaerocarpa Suppresses the Accumulation of Lipid Droplets in 3T3-L1 Adipocytes. ACS Food Sci. & Technol. 2: 1507-1516 Hayakawa, T., Suzuki, R., Kagotani, K., Okumura, K., and *Takebayashi, S. (2021) Camptothecin-induced replication stress alters the DNA replication landscape detected by E/L Repli-Seq. Cytogenet. Genome Res. 161: 437-444 *Takebayashi, S., Ryba, T., Wimbish, K., Hayakawa, T., Sakaue, M., Kuriya, K., Takahashi, S., Ogata, S., Hiratani, I., Okumura, K., Okano, M., and Ogata, M. (2021) The temporal order of DNA replication shaped by mammalian DNA methyltransferases. Cells 10: 266 Miura, H., Takahashi, S., Shibata, T., Nagao, K., Obuse, C., Okumura, K., Ogata, M., Hiratani, I., and *Takebayashi, S. (2020) Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing (scRepli-seq). Nat. Protoc. 15: 4058-4100 生野彰宏，南川佑太，竹林慎一郎，奥村克純（2021）葉酸欠乏によって起こる複製依存的DNA低メチル化とDNA損傷との関連性，日本食品化学学会誌，28巻: 23-32 Tanaka, H., Igata, T., Etoh, K., Koga, T., Takebayashi, S., and Nakao, M. (2020) The NSD2/WHSC1/MMSET methyltransferase prevents cellular senescence-associated epigenomic remodeling. Aging Cell 19: e13173 Ikuno, A., Akeda, K., Takebayashi, S., Shimaoka, M., Okumura, K., and Sudo, A. (2019) Genome-wide analysis of DNA methylation profile identifies differentially methylated loci associated with human intervertebral disc degeneration. PLoS One 14: e0222188 Miura, H., Takahashi, S., Poonperm, R., Tanigawa, A., Takebayashi, S., and Hiratani, I. (2019) Single-cell DNA replication profiling identifies spatiotemporal developmental dynamics of chromosome organization. Nat. Genet. 51: 1356-1368 Takahashi, S., Miura, H., Shibata, T., Nagao, K., Okumura, K., Ogata, M., Obuse, C., *Takebayashi, S., and Hiratani, I. (2019) Genome-wide stability of the DNA replication program in single mammalian cells. Nat. Genet. 51: 529-540 *Takebayashi, S., Ogata, S., Ogata, M. and Okumura, K. (2018) Mapping mammalian replication domains using the ion torrent semiconductor sequencing platform. Biosci. Biotechnol. Biochem. 82: 2098-2100 Hassan, W., Takebayashi, S., Abdalla, M. O., Fujino, K., Kudoh, S., Motooka, Y., Sato, Y., Naito, Y., Higaki, K., Wakimoto, J., Okada, S., Nakao, M., Ishikawa, Y., and Ito, T. (2017) Correlation between Histone Acetylation and Expression of Notch1 in Human Lung Carcinoma and Its Possible Role in Combined Small Cell Lung Carcinoma. Lab. Invest. 97:913-921 Tanaka, H., Takebayashi S. I., Sakamoto, A., Igata, T., Nakatsu, Y., Saitoh, N., Hino, S., and Nakao, M. (2017) The SETD8/PR-Set7 Methyltransferase Functions as a Barrier to Prevent Senescence-Associated Metabolic Remodeling. Cell Rep. 18:2148-2161. Kuriya, K., Higashiyama, E., Avşar-Ban, E., Okochi, N., Hattori, K., Ogata, S., Takebayashi, S. I., Ogata, M., Tamaru, Y., and Okumura, K. (2016) Direct visualization of replication dynamics in early zebrafish embryos. Biosci. Biotechnol. Biochem. 29:1-4. Kuriya, K., Higashiyama, E., Avşar-Ban, E., Tamaru, Y., Ogata, S., Takebayashi, S., Ogata, M., and Okumura, K. (2015) Direct Visualization of DNA Replication Dynamics in Zebrafish Cells. Zebrafish 12:432-439. Takebayashi, S., Tanaka, H., Hino, S., Nakatsu, Y., Igata, T., Sakamoto, A., Narita, M., and Nakao, M. (2015) Retinoblastoma protein promotes oxidative phosphorylation through upregulation of glycolytic genes in oncogene-induced senescent cells. Aging Cell 14:689-697. Takebayashi, S., Lei, I., Ryba, T., Sasaki, T., Dileep, V., Battaglia, D., Gao, X., Fang, P., Fan, Y., Esteban, M. A., Tang, J., Crabtree, G. R., Wang, Z. and Gilbert, D. M. (2013) Murine esBAF chromatin remodeling complex subunits BAF250a and Brg1 are necessary to maintain and reprogram pluripotency-specific replication timing of select replication domains. Epigenetics Chromatin. 6:42 Kirkland, B., Wang, Z., Zhang, P., Takebayashi, S., Lenhert, S., Gilbert, D. M. and Guan, J. (2013) Low-cost fabrication of centimetre-scale periodic arrays of single plasmid DNA molecules. Lab Chip.13:3367-3372. Liu, Y., Kirkland, B., Shirley, J., Wang, Z., Zhang, P., Stembridge, J., Wong, W., Takebayashi, S. I., Gilbert, D. M., Lenhert, S. and Guan, J. (2013) Development of a single-cell array for large-scale DNA fluorescence in situ hybridization. Lab Chip. 13: 1316-1324. Takebayashi, S., Dileep, V., Ryba, T., Dennis, J.H. and Gilbert, D.M. (2012) Chromatin-interaction compartment switch at developmentally regulated chromosomal domains reveals an unusual principle of chromatin folding. Proc. Natl. Acad. Sci. USA 109: 12574-12579. (featured in SnapShot: Replication timing. Cell 152, March 14, 2013) Wang, X., Takebayashi, S., Bernardin, E., Gilbert, D. M., Chella, R. and Guan, J. (2012) Microfluidic extraction and stretching of chromosomal DNA from single cell nuclei for DNA fluorescence in situ hybridization. Biomed. Microdevices 27: 443-451. Shirley, J.W., Ty, S., Takebayashi, S., Liu X., and Gilbert D.M. (2011) FISH Finder: a high-throughput tool for analyzing FISH images. Bioinformatics 27:933-938. Sugimura, K., Takebayashi, S., Taguchi, H., Takeda, S., and Okumura, K. (2008) PARP-1 ensures regulation of replication fork progression by homologous recombination on damaged DNA. J. Cell Biol. 183:1203-1212. Sharif, J., Muto, M., Takebayashi, S (first 3 authors contributed equally)., Suetake, I., Iwamatsu, A., Endo, T. A., Shinga, J., Mizutani-Koseki, Y., Toyoda, T., Okamura, K., Tajima, S., Mitsuya, K., Okano, M. and Koseki, H. (2007) The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature 450:908-912. Takebayashi, S., Tamura, T., Matsuoka, C. and Okano, M. (2007) Major and essential role for DNA methylation mark in mouse embryogenesis and stable association of Dnmt1 with newly replicated regions. Mol. Cell Biol. 27:8243-8258. Tsumura, A., Hayakawa, T., Kumaki, Y., Takebayashi, S., Sakaue, M., Matsuoka, C., Shimotohno, K., Ishikawa, F., Li, E., Ueda, H. R., Nakayama, J. and Okano, M. (2006) Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells 11:805-814. Takebayashi, S., Sugimura. K., Saito, T., Sato, C., Fukushima, Y., Taguchi, H. and Okumura, K. (2005) Regulation of replication at the R/G chromosomal band boundary and pericentromeric heterochromatin of mammalian cells. Exp. Cell Res. 304:162-174. Erven, A., Skynner, M.J., Okumura, K., Takebayashi, S., Brown, S. D., Steel, K. P. and Allen, N. D. (2002) A novel stereocilia defect in sensory hair cells of the deaf mouse mutant Tasmanian devil. Eur. J. Neurosci. 16:1433-1441. Kagotani, K., Takebayashi, S., Kohda, A., Taguchi, H., Paulsen, M., Walter, J., Reik, W. and Okumura, K. (2002) Replication timing properties within the mouse distal chromosome 7 imprinting cluster. Biosci. Biotechnol. Biochem. 66:1046-1051. Ito, M., Takebayashi, S., Okumura, K., Ohkubo, T., Nishio, M., Kawano, M., Komada, H., Ito, Y. and Tsurudome, M. (2002) Up-regulated expression of a novel gene in activated human peripheral blood mononuclear cells that is a truncated paralog of the human system L-amino acid transporter 1. Int. J. Biochem. Cell Biol. 34:1152-1163. Takebayashi, S. I., Manders, E. M., Kimura, H., Taguchi, H. and Okumura, K. (2001) Mapping sites where replication initiates in mammalian cells using DNA fibers. Exp. Cell Res. 271:263-268. Takebayashi, S., Nakao, M., Fujita, N., Sado, T., Tanaka, M., Taguchi, H. and Okumura, K. (2001) 5-Aza-2'-deoxycytidine induces histone hyperacetylation of mouse centromeric heterochromatin by a mechanism independent of DNA demethylation. Biochem. Biophys. Res. Commun. 288:921-926. Okano, M., Takebayashi, S., Okumura, K. and Li, E. (1999) Assignment of cytosine-5 DNA methyltransferases Dnmt3a and Dnmt3b to mouse chromosome bands 12A2-A3 and 2H1 by in situ hybridization. Cytogenet. Cell Genet. 86:333-334. Aso, T., Amimoto, K., Takebayashi, S., Okumura, K. and Hatakeyama, M. (1999) Structural organization and chromosome location of the mouse elongin A gene (Tceb3). Cytogenet. Cell Genet. 86:259-262. Hayashi, T., Nakamura, H., Okada, A., Takebayashi, S., Wakita, T., Yuasa, H., Okumura, K. and Suzuki, K. (1999) Organization and chromosomal localization of the human endothelial protein C receptor gene. Gene 238:367-373. Fujita, N., Takebayashi, S., Okumura, K., Kudo, S., Chiba, T., Saya, H. and Nakao, M. (1999) Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms. Mol. Cell Biol. 19:6415-6426. Koga, H., Matsui, S., Hirota, T., Takebayashi, S., Okumura, K. and Saya, H. (1999) A human homolog of Drosophila lethal(3)malignant brain tumor (l(3)mbt) protein associates with condensed mitotic chromosomes. Oncogene 18:3799-3809. Fujishige, K., Kotera, J., Michibata, H., Yuasa, K., Takebayashi, S., Okumura, K. and Omori, K. (1999) Cloning and characterization of a novel human phosphodiesterase that hydrolyzes both cAMP and cGMP (PDE10A). J. Biol. Chem. 274(26):18438-18445. Tsurudome, M., Ito, M., Takebayashi, S., Okumura, K., Nishio, M., Kawano, M., Kusagawa, S., Komada, H. and Ito, Y. (1999) Primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16. J. Immunol. 162:2462-2466. Yanaka, N., Kotera, J., Ohtsuka, A., Akatsuka, H., Imai, Y., Michibata, H., Fujishige, K., Kawai, E., Takebayashi, S., Okumura, K. and Omori, K. (1998) Expression, structure and chromosomal localization of the human cGMP-binding cGMP-specific phosphodiesterase PDE5A gene. Eur. J. Biochem. 255:391-399. （総説・著書） Sakamoto, M., Hori, S., Yamamoto, A., Yoneda, T., Kuriya K., and Takebayashi, S. (2022) scRepli-Seq: A Powerful Tool to Study Replication Timing and Genome Instability. Cytogenet. Genome Res. 162: 161-170 竹林慎一郎（2022）ハイブリダイゼーション. 遺伝学の百科事典・継承と多様性の源,日本遺伝学会 編, 丸善出版, pp.510-511 平谷伊智朗，竹林慎一郎 (2018) 複製タイミングと間期染色体構築．実験医学 36: 2933-2940 Takebayashi, S., Ogata, M., and Okumura, K. (2017) Anatomy of Mammalian Replication Domains. Genes (Basel). 8:E110. 竹林慎一郎、中尾光善「哺乳類遺伝子発現に重要な高次エピゲノム制御-機能単位としての染色体ドメイン構造とその調節」化学と生物、学会出版センター、pp597-599、2013 Takebayashi, S., Ryba, T. and Gilbert, D. M. (2012) Developmental control of replication timing defines a new breed of chromosomal domains with a novel mechanism of chromatin unfolding. Nucleus 3:500-507. Gilbert, D.M., Takebayashi, S.I., Ryba, T., Lu, J., Pope, B.D., Wilson, K.A. and Hiratani, I. (2011) Space and Time in the Nucleus: Developmental Control of Replication Timing and Chromosome Architecture. Cold Spring Harb. Symp. Quant. Biol. 75:143-153. Hiratani, I., Takebayashi, S., Lu, J. and Gilbert, D.M. (2009) Replication timing and transcriptional control: beyond cause and effect. Part II. CURR. OPIN. GEN. DEV. 19: 142-149. 竹林慎一郎「メチル化DNA維持機構の解明」化学と生物、学会出版センター、pp592-593、2009 武藤正弘，Sharif Jafar，竹林慎一郎「SRAドメインをもつNp95はメチル化 DNAにDnmt1をリクルートすることによりエピジェネティクな遺伝子発現の 維持を仲介する」実験医学、羊土社、pp921-925、2008 奥村克純，杉村和人，竹林慎一郎，齋藤辰朗「核内クロマチン構造と複製タイミング•フォークの制御」染色体サイクル制御の分子メカニズム、実験医学増刊、羊土社、pp739-746、2007 杉村和人，竹林慎一郎，奥村克純「DNAファイバーFISH-動物細胞ゲノムの複 製フォーク進行過程の解析」クロマチン・染色体実験プロトコール、羊土社、pp159-167、2004 岡野正樹，竹林慎一郎「エピジェネティクス」クロマチン構造と遺伝子機能制御、 シュプリンガーフェアラーク東京、pp205-211、2003 |
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